The 27-year-old rapper appeared to mock Jenner in his song "Playboy," which debuted Friday following his breakup with the 19-year-old reality star.
"She's a superstar / She got it bad for me / If I hang up, she call right back to me / You cut her legs off / She crawls right back to me," he raps on the track, which features Vince Staples.
Tyga and Jenner reportedly split again in April after two years of on-again, off-again dating.
"They tend to take little breaks all the time and then get back together," a source told People at the time. "It's definitely possible they'll work things out again."
"You know, I feel like [there was] a lot of drama, and it doesn't mean he's a bad person at all," the reality star said after host Andy Cohen asked her how "relieved" she was about the split.
"What was great was that it was so easy, that split. There has not been any drama with her since," she added.
Jenner was linked to rapper Travis Scott shortly after her breakup with Tyga. She was spotted with Scott at a Coachella party April 15, and celebrated the rapper's 25th birthday April 30 in New York.